Two cultures (10VL and 12VL) were obtained from Dysdercus sp. (fam. Pyrrhocoridae, Heteroptera), collected during the dry season on a vegetation along a small Rio Tarcolitos (left) near Parque Nacional Carara. The photo on the right shows the same site after a flood that occurred in June after the onset of the rain season.

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Isolate 10VL. Most cells have only a very short flagellum.

Typical promastigotes of the isolate 12VL, left - live cells with phase contrast, right - staining by Giemsa